Construct: shRNA TRCN0000264847

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172710.3-1895s21c1
DNA Barcode:
CTTCTATGAAACTGGATTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sel1l3 (231238)
Gene Description:
sel-1 suppressor of lin-12-like 3 (C. elegans)
Transcript:
RefSeq NM_172710.3 (CURRENT)
Match location:
Position 1895 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264847)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTATGAAACTGGATTAAA
Hairpin Sequence:
5'-CCGG-CTTCTATGAAACTGGATTAAA-CTCGAG-TTTAATCCAGTTTCATAGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTATGAAACTGGATTAAACTCGAGTTTAATCCAGTTTCATAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTATGAAACTGGATTAAACTCGAGTTTAATCCAGTTTCATAGAAG-3'

Other clones with same target sequence:

  1. TRCN0000216156