Construct: shRNA TRCN0000264858

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028791.5-3181s21c1
DNA Barcode:
GCTTTGGGTACTGGGTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cmtr1 (74157)
Gene Description:
cap methyltransferase 1
Transcript:
RefSeq NM_028791.5 (NON-CURRENT)
Match location:
Position 3181 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264858)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTTGGGTACTGGGTATAAT
Hairpin Sequence:
5'-CCGG-GCTTTGGGTACTGGGTATAAT-CTCGAG-ATTATACCCAGTACCCAAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTTGGGTACTGGGTATAATCTCGAGATTATACCCAGTACCCAAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTTGGGTACTGGGTATAATCTCGAGATTATACCCAGTACCCAAAGC-3'

Other clones with same target sequence:

(none)