Construct: shRNA TRCN0000264867

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026629.3-122s21c1
DNA Barcode:
TACGTACTGTGTCCGGACAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mturn (68235)
Gene Description:
maturin, neural progenitor differentiation regulator homolog (Xenopus)
Transcript:
RefSeq NM_026629.3 (NON-CURRENT)
Match location:
Position 122 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264867)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGTACTGTGTCCGGACAAC
Hairpin Sequence:
5'-CCGG-TACGTACTGTGTCCGGACAAC-CTCGAG-GTTGTCCGGACACAGTACGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGTACTGTGTCCGGACAACCTCGAGGTTGTCCGGACACAGTACGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGTACTGTGTCCGGACAACCTCGAGGTTGTCCGGACACAGTACGTA-3'

Other clones with same target sequence:

(none)