Construct: shRNA TRCN0000264871

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025722.2-358s21c1
DNA Barcode:
AGAAAGAGATCTCGAAGTAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4921524J17Rik (66714)
Gene Description:
RIKEN cDNA 4921524J17 gene
Transcript:
RefSeq NM_025722.2 (NON-CURRENT)
Match location:
Position 358 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264871)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAAGAGATCTCGAAGTAAC
Hairpin Sequence:
5'-CCGG-AGAAAGAGATCTCGAAGTAAC-CTCGAG-GTTACTTCGAGATCTCTTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAAGAGATCTCGAAGTAACCTCGAGGTTACTTCGAGATCTCTTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAAGAGATCTCGAAGTAACCTCGAGGTTACTTCGAGATCTCTTTCT-3'

Other clones with same target sequence:

(none)