Construct: shRNA TRCN0000264873

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025722.2-310s21c1
DNA Barcode:
GTTAGGCGAGAGAAGATAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
4921524J17Rik (66714)
Gene Description:
RIKEN cDNA 4921524J17 gene
Transcript:
RefSeq NM_025722.2 (NON-CURRENT)
Match location:
Position 310 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264873)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTAGGCGAGAGAAGATAAAT
Hairpin Sequence:
5'-CCGG-GTTAGGCGAGAGAAGATAAAT-CTCGAG-ATTTATCTTCTCTCGCCTAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTAGGCGAGAGAAGATAAATCTCGAGATTTATCTTCTCTCGCCTAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTAGGCGAGAGAAGATAAATCTCGAGATTTATCTTCTCTCGCCTAAC-3'

Other clones with same target sequence:

(none)