Construct: shRNA TRCN0000264936

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175313.4-694s21c1
DNA Barcode:
GGATTCCCACTGTCCATATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eogt (101351)
Gene Description:
EGF domain-specific O-linked N-acetylglucosamine (GlcNAc) transferase
Transcript:
RefSeq NM_175313.4 (CURRENT)
Match location:
Position 694 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264936)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGATTCCCACTGTCCATATAA
Hairpin Sequence:
5'-CCGG-GGATTCCCACTGTCCATATAA-CTCGAG-TTATATGGACAGTGGGAATCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGATTCCCACTGTCCATATAACTCGAGTTATATGGACAGTGGGAATCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGATTCCCACTGTCCATATAACTCGAGTTATATGGACAGTGGGAATCC-3'

Other clones with same target sequence:

  1. TRCN0000217396