Construct: shRNA TRCN0000264952

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053090.2-2054s21c1
DNA Barcode:
ACGTCCTCAGTGAGGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam126a (84652)
Gene Description:
family with sequence similarity 126, member A
Transcript:
RefSeq NM_053090.2 (NON-CURRENT)
Match location:
Position 2054 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264952)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGTCCTCAGTGAGGTTATTT
Hairpin Sequence:
5'-CCGG-ACGTCCTCAGTGAGGTTATTT-CTCGAG-AAATAACCTCACTGAGGACGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGTCCTCAGTGAGGTTATTTCTCGAGAAATAACCTCACTGAGGACGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGTCCTCAGTGAGGTTATTTCTCGAGAAATAACCTCACTGAGGACGT-3'

Other clones with same target sequence:

(none)