Construct: shRNA TRCN0000264970

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080995.1-1342s21c1
DNA Barcode:
CAGTATGATATCGCATGTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ddias (74041)
Gene Description:
DNA damage-induced apoptosis suppressor
Transcript:
RefSeq NM_001080995.1 (CURRENT)
Match location:
Position 1342 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264970)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGTATGATATCGCATGTTAC
Hairpin Sequence:
5'-CCGG-CAGTATGATATCGCATGTTAC-CTCGAG-GTAACATGCGATATCATACTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGTATGATATCGCATGTTACCTCGAGGTAACATGCGATATCATACTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGTATGATATCGCATGTTACCTCGAGGTAACATGCGATATCATACTG-3'

Other clones with same target sequence:

(none)