Construct: shRNA TRCN0000264989

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025806.1-460s21c1
DNA Barcode:
CTACCTCACTGCTCTACACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plbd1 (66857)
Gene Description:
phospholipase B domain containing 1
Transcript:
RefSeq NM_025806.1 (NON-CURRENT)
Match location:
Position 460 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264989)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACCTCACTGCTCTACACAT
Hairpin Sequence:
5'-CCGG-CTACCTCACTGCTCTACACAT-CTCGAG-ATGTGTAGAGCAGTGAGGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACCTCACTGCTCTACACATCTCGAGATGTGTAGAGCAGTGAGGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACCTCACTGCTCTACACATCTCGAGATGTGTAGAGCAGTGAGGTAG-3'

Other clones with same target sequence:

(none)