Construct: shRNA TRCN0000265009

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133778.2-345s21c1
DNA Barcode:
ATCGTCCCTGCATGGTATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam131a (78408)
Gene Description:
family with sequence similarity 131, member A
Transcript:
RefSeq NM_133778.2 (CURRENT)
Match location:
Position 345 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265009)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGTCCCTGCATGGTATTTC
Hairpin Sequence:
5'-CCGG-ATCGTCCCTGCATGGTATTTC-CTCGAG-GAAATACCATGCAGGGACGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGTCCCTGCATGGTATTTCCTCGAGGAAATACCATGCAGGGACGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGTCCCTGCATGGTATTTCCTCGAGGAAATACCATGCAGGGACGAT-3'

Other clones with same target sequence:

(none)