Construct: shRNA TRCN0000265017

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026622.3-1713s21c1
DNA Barcode:
TTACAAACTGTGGATACATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abhd18 (269423)
Gene Description:
abhydrolase domain containing 18
Transcript:
RefSeq NM_026622.3 (CURRENT)
Match location:
Position 1713 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265017)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACAAACTGTGGATACATTT
Hairpin Sequence:
5'-CCGG-TTACAAACTGTGGATACATTT-CTCGAG-AAATGTATCCACAGTTTGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACAAACTGTGGATACATTTCTCGAGAAATGTATCCACAGTTTGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACAAACTGTGGATACATTTCTCGAGAAATGTATCCACAGTTTGTAA-3'

Other clones with same target sequence:

(none)