Construct: shRNA TRCN0000265063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172615.3-1384s21c1
DNA Barcode:
GGACAAAGAAGAGCCATATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
1700021K19Rik (224118)
Gene Description:
RIKEN cDNA 1700021K19 gene
Transcript:
RefSeq NM_172615.3 (NON-CURRENT)
Match location:
Position 1384 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACAAAGAAGAGCCATATTC
Hairpin Sequence:
5'-CCGG-GGACAAAGAAGAGCCATATTC-CTCGAG-GAATATGGCTCTTCTTTGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACAAAGAAGAGCCATATTCCTCGAGGAATATGGCTCTTCTTTGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACAAAGAAGAGCCATATTCCTCGAGGAATATGGCTCTTCTTTGTCC-3'

Other clones with same target sequence:

(none)