Construct: shRNA TRCN0000265092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029920.5-1034s21c1
DNA Barcode:
AGATACAAATGCCAATCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mtus2 (77521)
Gene Description:
microtubule associated tumor suppressor candidate 2
Transcript:
RefSeq NM_029920.5 (NON-CURRENT)
Match location:
Position 1034 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATACAAATGCCAATCAAAT
Hairpin Sequence:
5'-CCGG-AGATACAAATGCCAATCAAAT-CTCGAG-ATTTGATTGGCATTTGTATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATACAAATGCCAATCAAATCTCGAGATTTGATTGGCATTTGTATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATACAAATGCCAATCAAATCTCGAGATTTGATTGGCATTTGTATCT-3'

Other clones with same target sequence:

(none)