Construct: shRNA TRCN0000265127

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028047.2-1105s21c1
DNA Barcode:
TTGGACCACCTCATCAATAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smg9 (71997)
Gene Description:
smg-9 homolog, nonsense mediated mRNA decay factor (C. elegans)
Transcript:
RefSeq NM_028047.2 (CURRENT)
Match location:
Position 1105 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265127)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGACCACCTCATCAATAAC
Hairpin Sequence:
5'-CCGG-TTGGACCACCTCATCAATAAC-CTCGAG-GTTATTGATGAGGTGGTCCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGACCACCTCATCAATAACCTCGAGGTTATTGATGAGGTGGTCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGACCACCTCATCAATAACCTCGAGGTTATTGATGAGGTGGTCCAA-3'

Other clones with same target sequence:

(none)