Construct: shRNA TRCN0000265134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030728.3-3936s21c1
DNA Barcode:
TCGGAATGACTTCGCTTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cemip (80982)
Gene Description:
cell migration inducing protein, hyaluronan binding
Transcript:
RefSeq NM_030728.3 (NON-CURRENT)
Match location:
Position 3936 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGGAATGACTTCGCTTATAT
Hairpin Sequence:
5'-CCGG-TCGGAATGACTTCGCTTATAT-CTCGAG-ATATAAGCGAAGTCATTCCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGGAATGACTTCGCTTATATCTCGAGATATAAGCGAAGTCATTCCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGGAATGACTTCGCTTATATCTCGAGATATAAGCGAAGTCATTCCGA-3'

Other clones with same target sequence:

(none)