Construct: shRNA TRCN0000265165

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001042714.1-368s21c1
DNA Barcode:
GATCATGCTCATGCTAGTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ankdd1b (271144)
Gene Description:
ankyrin repeat and death domain containing 1B
Transcript:
RefSeq NM_001042714.1 (CURRENT)
Match location:
Position 368 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265165)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCATGCTCATGCTAGTTAA
Hairpin Sequence:
5'-CCGG-GATCATGCTCATGCTAGTTAA-CTCGAG-TTAACTAGCATGAGCATGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCATGCTCATGCTAGTTAACTCGAGTTAACTAGCATGAGCATGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCATGCTCATGCTAGTTAACTCGAGTTAACTAGCATGAGCATGATC-3'

Other clones with same target sequence:

(none)