Construct: shRNA TRCN0000265190

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033405.2-985s21c1
DNA Barcode:
TTCACATCTGACGTGACTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Treml2 (328833)
Gene Description:
triggering receptor expressed on myeloid cells-like 2
Transcript:
RefSeq NM_001033405.2 (CURRENT)
Match location:
Position 985 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265190)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCACATCTGACGTGACTATG
Hairpin Sequence:
5'-CCGG-TTCACATCTGACGTGACTATG-CTCGAG-CATAGTCACGTCAGATGTGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCACATCTGACGTGACTATGCTCGAGCATAGTCACGTCAGATGTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCACATCTGACGTGACTATGCTCGAGCATAGTCACGTCAGATGTGAA-3'

Other clones with same target sequence:

(none)