Construct: shRNA TRCN0000265248

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029557.1-1360s21c1
DNA Barcode:
GCAGATCAGCTTCGATGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tsen54 (76265)
Gene Description:
tRNA splicing endonuclease subunit 54
Transcript:
RefSeq NM_029557.1 (CURRENT)
Match location:
Position 1360 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265248)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATCAGCTTCGATGTTTA
Hairpin Sequence:
5'-CCGG-GCAGATCAGCTTCGATGTTTA-CTCGAG-TAAACATCGAAGCTGATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATCAGCTTCGATGTTTACTCGAGTAAACATCGAAGCTGATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATCAGCTTCGATGTTTACTCGAGTAAACATCGAAGCTGATCTGC-3'

Other clones with same target sequence:

(none)