Construct: shRNA TRCN0000265290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173393.1-1359s21c1
DNA Barcode:
GAGGCTGCGGAGGACTATTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Xkr6 (219149)
Gene Description:
X-linked Kx blood group related 6
Transcript:
RefSeq NM_173393.1 (NON-CURRENT)
Match location:
Position 1359 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCTGCGGAGGACTATTAA
Hairpin Sequence:
5'-CCGG-GAGGCTGCGGAGGACTATTAA-CTCGAG-TTAATAGTCCTCCGCAGCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCTGCGGAGGACTATTAACTCGAGTTAATAGTCCTCCGCAGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCTGCGGAGGACTATTAACTCGAGTTAATAGTCCTCCGCAGCCTC-3'

Other clones with same target sequence:

(none)