Construct: shRNA TRCN0000265314

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146108.1-249s21c1
DNA Barcode:
ATATGATCCGGCAGATCTATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hibch (227095)
Gene Description:
3-hydroxyisobutyryl-Coenzyme A hydrolase
Transcript:
RefSeq NM_146108.1 (NON-CURRENT)
Match location:
Position 249 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265314)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATGATCCGGCAGATCTATC
Hairpin Sequence:
5'-CCGG-ATATGATCCGGCAGATCTATC-CTCGAG-GATAGATCTGCCGGATCATAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATGATCCGGCAGATCTATCCTCGAGGATAGATCTGCCGGATCATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATGATCCGGCAGATCTATCCTCGAGGATAGATCTGCCGGATCATAT-3'

Other clones with same target sequence:

(none)