Construct: shRNA TRCN0000265344

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001122675.1-2616s21c1
DNA Barcode:
CCCTAATGCGGTGGCTATTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zcchc2 (227449)
Gene Description:
zinc finger, CCHC domain containing 2
Transcript:
RefSeq NM_001122675.1 (CURRENT)
Match location:
Position 2616 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265344)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAATGCGGTGGCTATTTC
Hairpin Sequence:
5'-CCGG-CCCTAATGCGGTGGCTATTTC-CTCGAG-GAAATAGCCACCGCATTAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAATGCGGTGGCTATTTCCTCGAGGAAATAGCCACCGCATTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAATGCGGTGGCTATTTCCTCGAGGAAATAGCCACCGCATTAGGG-3'

Other clones with same target sequence:

(none)