Construct: shRNA TRCN0000265383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001083342.1-1003s21c1
DNA Barcode:
ATCCACGAGATCGAGCGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Disp3 (242748)
Gene Description:
dispatched RND transporter family member 3
Transcript:
RefSeq NM_001083342.1 (CURRENT)
Match location:
Position 1003 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCACGAGATCGAGCGTAAA
Hairpin Sequence:
5'-CCGG-ATCCACGAGATCGAGCGTAAA-CTCGAG-TTTACGCTCGATCTCGTGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCACGAGATCGAGCGTAAACTCGAGTTTACGCTCGATCTCGTGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCACGAGATCGAGCGTAAACTCGAGTTTACGCTCGATCTCGTGGAT-3'

Other clones with same target sequence:

(none)