Construct: shRNA TRCN0000265389

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033296.2-1531s21c1
DNA Barcode:
ACGGCCTATTTCGCCTATAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sel1l2 (228684)
Gene Description:
sel-1 suppressor of lin-12-like 2 (C. elegans)
Transcript:
RefSeq NM_001033296.2 (CURRENT)
Match location:
Position 1531 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265389)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGCCTATTTCGCCTATAAG
Hairpin Sequence:
5'-CCGG-ACGGCCTATTTCGCCTATAAG-CTCGAG-CTTATAGGCGAAATAGGCCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGCCTATTTCGCCTATAAGCTCGAGCTTATAGGCGAAATAGGCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGCCTATTTCGCCTATAAGCTCGAGCTTATAGGCGAAATAGGCCGT-3'

Other clones with same target sequence:

(none)