Construct: shRNA TRCN0000265466

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001040026.1-436s21c1
DNA Barcode:
CTACCTGGGTCAATGGGTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sco1 (52892)
Gene Description:
SCO1 cytochrome c oxidase assembly protein
Transcript:
RefSeq NM_001040026.1 (CURRENT)
Match location:
Position 436 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265466)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACCTGGGTCAATGGGTATT
Hairpin Sequence:
5'-CCGG-CTACCTGGGTCAATGGGTATT-CTCGAG-AATACCCATTGACCCAGGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACCTGGGTCAATGGGTATTCTCGAGAATACCCATTGACCCAGGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACCTGGGTCAATGGGTATTCTCGAGAATACCCATTGACCCAGGTAG-3'

Other clones with same target sequence:

(none)