Construct: shRNA TRCN0000265500

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175089.3-1759s21c1
DNA Barcode:
AGCGGTTGGAGAGGATAAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nek1 (18004)
Gene Description:
NIMA (never in mitosis gene a)-related expressed kinase 1
Transcript:
RefSeq NM_175089.3 (NON-CURRENT)
Match location:
Position 1759 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265500)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGGTTGGAGAGGATAAATA
Hairpin Sequence:
5'-CCGG-AGCGGTTGGAGAGGATAAATA-CTCGAG-TATTTATCCTCTCCAACCGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGGTTGGAGAGGATAAATACTCGAGTATTTATCCTCTCCAACCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGGTTGGAGAGGATAAATACTCGAGTATTTATCCTCTCCAACCGCT-3'

Other clones with same target sequence:

(none)