Construct: shRNA TRCN0000265505

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024562.1-1969s21c1
DNA Barcode:
AGAATGTCTGAGCAGATATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TANGO6 (79613)
Gene Description:
transport and golgi organization 6 homolog
Transcript:
RefSeq NM_024562.1 (NON-CURRENT)
Match location:
Position 1969 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265505)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATGTCTGAGCAGATATTC
Hairpin Sequence:
5'-CCGG-AGAATGTCTGAGCAGATATTC-CTCGAG-GAATATCTGCTCAGACATTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATGTCTGAGCAGATATTCCTCGAGGAATATCTGCTCAGACATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATGTCTGAGCAGATATTCCTCGAGGAATATCTGCTCAGACATTCT-3'

Other clones with same target sequence:

(none)