Construct: shRNA TRCN0000265528

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001082412.2-1360s21c1
DNA Barcode:
TGGTGTCTGGTAGTGTCAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Slc25a53 (67062)
Gene Description:
solute carrier family 25, member 53
Transcript:
RefSeq NM_001082412.2 (CURRENT)
Match location:
Position 1360 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265528)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGTCTGGTAGTGTCAATG
Hairpin Sequence:
5'-CCGG-TGGTGTCTGGTAGTGTCAATG-CTCGAG-CATTGACACTACCAGACACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGTCTGGTAGTGTCAATGCTCGAGCATTGACACTACCAGACACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGTCTGGTAGTGTCAATGCTCGAGCATTGACACTACCAGACACCA-3'

Other clones with same target sequence:

(none)