Construct: shRNA TRCN0000265576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033489.1-2100s21c1
DNA Barcode:
TGTAGAACTATACCCGATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rnf207 (433809)
Gene Description:
ring finger protein 207
Transcript:
RefSeq NM_001033489.1 (NON-CURRENT)
Match location:
Position 2100 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTAGAACTATACCCGATAAT
Hairpin Sequence:
5'-CCGG-TGTAGAACTATACCCGATAAT-CTCGAG-ATTATCGGGTATAGTTCTACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTAGAACTATACCCGATAATCTCGAGATTATCGGGTATAGTTCTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTAGAACTATACCCGATAATCTCGAGATTATCGGGTATAGTTCTACA-3'

Other clones with same target sequence:

(none)