Construct: shRNA TRCN0000265648

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178628.4-1145s21c1
DNA Barcode:
GAGTCCTGAGCGTCTAGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atl1 (73991)
Gene Description:
atlastin GTPase 1
Transcript:
RefSeq NM_178628.4 (NON-CURRENT)
Match location:
Position 1145 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265648)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTCCTGAGCGTCTAGATAT
Hairpin Sequence:
5'-CCGG-GAGTCCTGAGCGTCTAGATAT-CTCGAG-ATATCTAGACGCTCAGGACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTCCTGAGCGTCTAGATATCTCGAGATATCTAGACGCTCAGGACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTCCTGAGCGTCTAGATATCTCGAGATATCTAGACGCTCAGGACTC-3'

Other clones with same target sequence:

(none)