Construct: shRNA TRCN0000265649

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000218.2-533s21c1
DNA Barcode:
TGTCCACCATCGAGCAGTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNQ1 (3784)
Gene Description:
potassium voltage-gated channel subfamily Q member 1
Transcript:
RefSeq NM_000218.2 (NON-CURRENT)
Match location:
Position 533 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265649)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCCACCATCGAGCAGTATG
Hairpin Sequence:
5'-CCGG-TGTCCACCATCGAGCAGTATG-CTCGAG-CATACTGCTCGATGGTGGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCCACCATCGAGCAGTATGCTCGAGCATACTGCTCGATGGTGGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCCACCATCGAGCAGTATGCTCGAGCATACTGCTCGATGGTGGACA-3'

Other clones with same target sequence:

(none)