Construct: shRNA TRCN0000265723

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010367.2-2201s21c1
DNA Barcode:
TTGACCCGGATGACCCTAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Magi1 (14924)
Gene Description:
membrane associated guanylate kinase, WW and PDZ domain containing 1
Transcript:
RefSeq NM_010367.2 (NON-CURRENT)
Match location:
Position 2201 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265723)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGACCCGGATGACCCTAATA
Hairpin Sequence:
5'-CCGG-TTGACCCGGATGACCCTAATA-CTCGAG-TATTAGGGTCATCCGGGTCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGACCCGGATGACCCTAATACTCGAGTATTAGGGTCATCCGGGTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGACCCGGATGACCCTAATACTCGAGTATTAGGGTCATCCGGGTCAA-3'

Other clones with same target sequence:

(none)