Construct: shRNA TRCN0000265724
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010367.2-5621s21c1
- DNA Barcode:
- AGCCGAGTAGATCCGAATATA
Original Target:
- Taxon:
- Mus musculus (mouse)
- Gene:
- Magi1 (14924)
- Gene Description:
- membrane associated guanylate kinase, WW and PDZ domain containing 1
- Transcript:
- RefSeq NM_010367.2 (NON-CURRENT)
- Match location:
- Position 5621 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000265724)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
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Sequence Information
- Target Sequence:
- AGCCGAGTAGATCCGAATATA
- Hairpin Sequence:
- 5'-CCGG-AGCCGAGTAGATCCGAATATA-CTCGAG-TATATTCGGATCTACTCGGCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGCCGAGTAGATCCGAATATACTCGAGTATATTCGGATCTACTCGGCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGCCGAGTAGATCCGAATATACTCGAGTATATTCGGATCTACTCGGCT-3'