Construct: shRNA TRCN0000265751

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207705.1-1317s21c1
DNA Barcode:
ATGTATACCAAGGACTATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Elmo2 (140579)
Gene Description:
engulfment and cell motility 2
Transcript:
RefSeq NM_207705.1 (NON-CURRENT)
Match location:
Position 1317 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265751)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTATACCAAGGACTATAAA
Hairpin Sequence:
5'-CCGG-ATGTATACCAAGGACTATAAA-CTCGAG-TTTATAGTCCTTGGTATACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTATACCAAGGACTATAAACTCGAGTTTATAGTCCTTGGTATACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTATACCAAGGACTATAAACTCGAGTTTATAGTCCTTGGTATACAT-3'

Other clones with same target sequence:

(none)