Construct: shRNA TRCN0000265833

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_058197.3-950s21c1
DNA Barcode:
CCGATTGAAAGAACCAGAGAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CDKN2A (1029)
Gene Description:
cyclin dependent kinase inhibitor 2A
Transcript:
RefSeq NM_058197.3 (NON-CURRENT)
Match location:
Position 950 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265833)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGATTGAAAGAACCAGAGAG
Hairpin Sequence:
5'-CCGG-CCGATTGAAAGAACCAGAGAG-CTCGAG-CTCTCTGGTTCTTTCAATCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGATTGAAAGAACCAGAGAGCTCGAGCTCTCTGGTTCTTTCAATCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGATTGAAAGAACCAGAGAGCTCGAGCTCTCTGGTTCTTTCAATCGG-3'

Other clones with same target sequence:

(none)