Construct: shRNA TRCN0000266767

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029714.3-2435s21c1
DNA Barcode:
ATAGCCAAGGACCCACTATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Catsperg2 (76718)
Gene Description:
cation channel sperm associated auxiliary subunit gamma 2
Transcript:
RefSeq NM_029714.3 (CURRENT)
Match location:
Position 2435 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000266767)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAGCCAAGGACCCACTATTA
Hairpin Sequence:
5'-CCGG-ATAGCCAAGGACCCACTATTA-CTCGAG-TAATAGTGGGTCCTTGGCTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAGCCAAGGACCCACTATTACTCGAGTAATAGTGGGTCCTTGGCTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAGCCAAGGACCCACTATTACTCGAGTAATAGTGGGTCCTTGGCTAT-3'

Other clones with same target sequence:

(none)