Construct: shRNA TRCN0000266879

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177173.4-1772s21c1
DNA Barcode:
CCTGTCCTACCCTGATCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
A830018L16Rik (320492)
Gene Description:
RIKEN cDNA A830018L16 gene
Transcript:
RefSeq NM_177173.4 (NON-CURRENT)
Match location:
Position 1772 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000266879)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGTCCTACCCTGATCTATT
Hairpin Sequence:
5'-CCGG-CCTGTCCTACCCTGATCTATT-CTCGAG-AATAGATCAGGGTAGGACAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGTCCTACCCTGATCTATTCTCGAGAATAGATCAGGGTAGGACAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGTCCTACCCTGATCTATTCTCGAGAATAGATCAGGGTAGGACAGG-3'

Other clones with same target sequence:

(none)