Construct: shRNA TRCN0000266893

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025556.2-447s21c1
DNA Barcode:
ACCACAGGGAGACATGATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Coprs (66423)
Gene Description:
coordinator of PRMT5, differentiation stimulator
Transcript:
RefSeq NM_025556.2 (NON-CURRENT)
Match location:
Position 447 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000266893)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCACAGGGAGACATGATATA
Hairpin Sequence:
5'-CCGG-ACCACAGGGAGACATGATATA-CTCGAG-TATATCATGTCTCCCTGTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCACAGGGAGACATGATATACTCGAGTATATCATGTCTCCCTGTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCACAGGGAGACATGATATACTCGAGTATATCATGTCTCCCTGTGGT-3'

Other clones with same target sequence:

(none)