Construct: shRNA TRCN0000266956

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029841.2-1298s21c1
DNA Barcode:
TGGAGGCAACGCTCAACTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2510039O18Rik (77034)
Gene Description:
RIKEN cDNA 2510039O18 gene
Transcript:
RefSeq NM_029841.2 (NON-CURRENT)
Match location:
Position 1298 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000266956)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAGGCAACGCTCAACTATG
Hairpin Sequence:
5'-CCGG-TGGAGGCAACGCTCAACTATG-CTCGAG-CATAGTTGAGCGTTGCCTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAGGCAACGCTCAACTATGCTCGAGCATAGTTGAGCGTTGCCTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAGGCAACGCTCAACTATGCTCGAGCATAGTTGAGCGTTGCCTCCA-3'

Other clones with same target sequence:

(none)