Construct: shRNA TRCN0000266995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172652.3-1321s21c1
DNA Barcode:
CTCCAGTCCTCTTCGTCATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kansl3 (226976)
Gene Description:
KAT8 regulatory NSL complex subunit 3
Transcript:
RefSeq NM_172652.3 (CURRENT)
Match location:
Position 1321 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000266995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCAGTCCTCTTCGTCATTG
Hairpin Sequence:
5'-CCGG-CTCCAGTCCTCTTCGTCATTG-CTCGAG-CAATGACGAAGAGGACTGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCAGTCCTCTTCGTCATTGCTCGAGCAATGACGAAGAGGACTGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCAGTCCTCTTCGTCATTGCTCGAGCAATGACGAAGAGGACTGGAG-3'

Other clones with same target sequence:

(none)