Construct: shRNA TRCN0000267142

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177658.3-4570s21c1
DNA Barcode:
TGGTGAGGCAGACGGTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ralgapb (228850)
Gene Description:
Ral GTPase activating protein, beta subunit (non-catalytic)
Transcript:
RefSeq NM_177658.3 (NON-CURRENT)
Match location:
Position 4570 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267142)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGTGAGGCAGACGGTAATAA
Hairpin Sequence:
5'-CCGG-TGGTGAGGCAGACGGTAATAA-CTCGAG-TTATTACCGTCTGCCTCACCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGTGAGGCAGACGGTAATAACTCGAGTTATTACCGTCTGCCTCACCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGTGAGGCAGACGGTAATAACTCGAGTTATTACCGTCTGCCTCACCA-3'

Other clones with same target sequence:

(none)