Construct: shRNA TRCN0000267178

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081314.1-2023s21c1
DNA Barcode:
ACCAGGACTGCTGCTTCGATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C2cd4b (75697)
Gene Description:
C2 calcium-dependent domain containing 4B
Transcript:
RefSeq NM_001081314.1 (NON-CURRENT)
Match location:
Position 2023 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267178)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAGGACTGCTGCTTCGATC
Hairpin Sequence:
5'-CCGG-ACCAGGACTGCTGCTTCGATC-CTCGAG-GATCGAAGCAGCAGTCCTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAGGACTGCTGCTTCGATCCTCGAGGATCGAAGCAGCAGTCCTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAGGACTGCTGCTTCGATCCTCGAGGATCGAAGCAGCAGTCCTGGT-3'

Other clones with same target sequence:

(none)