Construct: shRNA TRCN0000267197

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081022.1-5627s21c1
DNA Barcode:
AGTCTCCCAGTAGTTGGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
D430042O09Rik (233865)
Gene Description:
RIKEN cDNA D430042O09 gene
Transcript:
RefSeq NM_001081022.1 (CURRENT)
Match location:
Position 5627 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267197)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCTCCCAGTAGTTGGTAAT
Hairpin Sequence:
5'-CCGG-AGTCTCCCAGTAGTTGGTAAT-CTCGAG-ATTACCAACTACTGGGAGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCTCCCAGTAGTTGGTAATCTCGAGATTACCAACTACTGGGAGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCTCCCAGTAGTTGGTAATCTCGAGATTACCAACTACTGGGAGACT-3'

Other clones with same target sequence:

(none)