Construct: shRNA TRCN0000267228

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001034874.2-1508s21c1
DNA Barcode:
TCTCGCATACGGACGTCTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shisa6 (380702)
Gene Description:
shisa family member 6
Transcript:
RefSeq NM_001034874.2 (NON-CURRENT)
Match location:
Position 1508 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267228)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTCGCATACGGACGTCTTTG
Hairpin Sequence:
5'-CCGG-TCTCGCATACGGACGTCTTTG-CTCGAG-CAAAGACGTCCGTATGCGAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTCGCATACGGACGTCTTTGCTCGAGCAAAGACGTCCGTATGCGAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTCGCATACGGACGTCTTTGCTCGAGCAAAGACGTCCGTATGCGAGA-3'

Other clones with same target sequence:

(none)