Construct: shRNA TRCN0000267231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001143671.1-332s21c1
DNA Barcode:
GGCTACTACCCAGGGATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tmem255b (272465)
Gene Description:
transmembrane protein 255B
Transcript:
RefSeq NM_001143671.1 (CURRENT)
Match location:
Position 332 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCTACTACCCAGGGATTATT
Hairpin Sequence:
5'-CCGG-GGCTACTACCCAGGGATTATT-CTCGAG-AATAATCCCTGGGTAGTAGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCTACTACCCAGGGATTATTCTCGAGAATAATCCCTGGGTAGTAGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCTACTACCCAGGGATTATTCTCGAGAATAATCCCTGGGTAGTAGCC-3'

Other clones with same target sequence:

(none)