Construct: shRNA TRCN0000267281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001045526.2-556s21c1
DNA Barcode:
CTCTAGAATAGCCGGATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scimp (327957)
Gene Description:
SLP adaptor and CSK interacting membrane protein
Transcript:
RefSeq NM_001045526.2 (CURRENT)
Match location:
Position 556 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTAGAATAGCCGGATTATA
Hairpin Sequence:
5'-CCGG-CTCTAGAATAGCCGGATTATA-CTCGAG-TATAATCCGGCTATTCTAGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTAGAATAGCCGGATTATACTCGAGTATAATCCGGCTATTCTAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTAGAATAGCCGGATTATACTCGAGTATAATCCGGCTATTCTAGAG-3'

Other clones with same target sequence:

(none)