Construct: shRNA TRCN0000267421

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081344.2-2365s21c1
DNA Barcode:
ATCGAACTTTACGGCTCAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Stxbp5 (78808)
Gene Description:
syntaxin binding protein 5 (tomosyn)
Transcript:
RefSeq NM_001081344.2 (CURRENT)
Match location:
Position 2365 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267421)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCGAACTTTACGGCTCAAAT
Hairpin Sequence:
5'-CCGG-ATCGAACTTTACGGCTCAAAT-CTCGAG-ATTTGAGCCGTAAAGTTCGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCGAACTTTACGGCTCAAATCTCGAGATTTGAGCCGTAAAGTTCGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCGAACTTTACGGCTCAAATCTCGAGATTTGAGCCGTAAAGTTCGAT-3'

Other clones with same target sequence:

(none)