Construct: shRNA TRCN0000267454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004156.2-2885s21c1
DNA Barcode:
AGGCTAGCCTCTTACAATTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plekhg5 (269608)
Gene Description:
pleckstrin homology domain containing, family G (with RhoGef domain) member 5
Transcript:
RefSeq NM_001004156.2 (NON-CURRENT)
Match location:
Position 2885 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCTAGCCTCTTACAATTAC
Hairpin Sequence:
5'-CCGG-AGGCTAGCCTCTTACAATTAC-CTCGAG-GTAATTGTAAGAGGCTAGCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCTAGCCTCTTACAATTACCTCGAGGTAATTGTAAGAGGCTAGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCTAGCCTCTTACAATTACCTCGAGGTAATTGTAAGAGGCTAGCCT-3'

Other clones with same target sequence:

(none)