Construct: shRNA TRCN0000267455

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026672.2-757s21c1
DNA Barcode:
CAGGGTGGGCTGTAGGATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gstm7 (68312)
Gene Description:
glutathione S-transferase, mu 7
Transcript:
RefSeq NM_026672.2 (NON-CURRENT)
Match location:
Position 757 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267455)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGGTGGGCTGTAGGATAAA
Hairpin Sequence:
5'-CCGG-CAGGGTGGGCTGTAGGATAAA-CTCGAG-TTTATCCTACAGCCCACCCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGGTGGGCTGTAGGATAAACTCGAGTTTATCCTACAGCCCACCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGGTGGGCTGTAGGATAAACTCGAGTTTATCCTACAGCCCACCCTG-3'

Other clones with same target sequence:

(none)