Construct: shRNA TRCN0000267496

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024678.2-879s21c1
DNA Barcode:
ACAGCAGCCTCATCTGCATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRC24 (441381)
Gene Description:
leucine rich repeat containing 24
Transcript:
RefSeq NM_001024678.2 (NON-CURRENT)
Match location:
Position 879 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267496)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGCAGCCTCATCTGCATTC
Hairpin Sequence:
5'-CCGG-ACAGCAGCCTCATCTGCATTC-CTCGAG-GAATGCAGATGAGGCTGCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGCAGCCTCATCTGCATTCCTCGAGGAATGCAGATGAGGCTGCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGCAGCCTCATCTGCATTCCTCGAGGAATGCAGATGAGGCTGCTGT-3'

Other clones with same target sequence:

(none)