Construct: shRNA TRCN0000267595

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081267.1-3845s21c1
DNA Barcode:
CCGAAGCACAGATGACTATTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rsf1 (233532)
Gene Description:
remodeling and spacing factor 1
Transcript:
RefSeq NM_001081267.1 (NON-CURRENT)
Match location:
Position 3845 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267595)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAAGCACAGATGACTATTC
Hairpin Sequence:
5'-CCGG-CCGAAGCACAGATGACTATTC-CTCGAG-GAATAGTCATCTGTGCTTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAAGCACAGATGACTATTCCTCGAGGAATAGTCATCTGTGCTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAAGCACAGATGACTATTCCTCGAGGAATAGTCATCTGTGCTTCGG-3'

Other clones with same target sequence:

(none)